Transglutaminase activity in pressure-induced gelation assisted by prior setting

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High Pressure Effects on Gelation of Surimi and Turkey Breast Muscle Enhanced by Microbial Transglutaminase

High pressure effects on the strength (stress) and elasticity/ deformability (strain) of surimi and turkey breast meat gels containing microbial transglutaminase (TGase) were evaluated. Pressurization of muscle proteins at 4oC prior to incubation at 25°C or 40°C (setting) increased gel strength 2–3 fold in uncooked surimi gels, but not in uncooked turkey gels. However, pressurization at 40°C or...

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Coagulation and Gelation of Soy Protein Isolates Induced by Microbial Transglutaminase

The reaction process and corresponding mechanism of coagulation and gelation of native soy protein isolates (SPIs) induced by microbial transglutaminase (MTGase) were investigated. The protein constituents of SPIs, including a majority of subunits of b-conglycinin and acidic subunits of glycinin, could be polymerized by MTGase to form high weight molecular (WM) biopolymers. Both the coagulation...

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Microwave-assisted cross-linking of milk proteins induced by microbial transglutaminase

We investigated the combined effects of microbial transglutaminase (MTGase, 7.0 units/mL) and microwave irradiation (MI) on the polymerization of milk proteins at 30 °C for 3 h. The addition of MTGase caused the milk proteins to become polymerized, which resulted in the formation of components with a higher molecular-weight (>130 kDa). SDS-PAGE analysis revealed reductions in the protein conten...

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Tissue Transglutaminase Elevates Intraocular Pressure in Mice.

Purpose Tissue transglutaminase (TGM2) is elevated in glaucomatous trabecular meshwork (TM) tissues. We investigated whether increased expression of TGM2 increases extracellular matrix crosslinking in the TM, thereby increasing aqueous humor outflow resistance and elevating intraocular pressure (IOP) in mouse eyes. Methods GTM3, primary human GTM 125-05, and cultured mouse TM cells were trans...

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Transglutaminase Activity in Alzheimer’s Disease

Cover image: immunofluorescence staining of tTG (green) and tTG activity (red) in a brain blood vessel of the APP23 Alzheimer mouse model as used in Chapter 5. APP23 mice are originally from healthcare company Novartis. Image printed with permission of Novartis. Chapter images: immunofluorescence stainings of collagen (green) in culture plates after removal of cells, as described in Chapter 4.

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ژورنال

عنوان ژورنال: Food Chemistry

سال: 2005

ISSN: 0308-8146

DOI: 10.1016/j.foodchem.2004.05.022